Bridget Phillipson has been accused of delaying the publishing of guidance on single-sex spaces amid concerns she may lose support from Labour backbenchers.
The Equality and Human Rights Commission (EHRC) gave the statutory guidance to Mrs Phillipson, who is also the Women and Equalities Minister.
The document sets out how gyms, clubs and hospitals must judge single-sex spaces based on biology.
While it has not yet been laid in parliament, and there has been intense backlash from transgender campaigners and opposition MPs.
Sources close to Mrs Phillipson accused her opponents “weaponising gender issues” in a “cynical and divisive” way and the Government was “taking the time” to get the guidance right.
One told The Times: “To suggest that the timing of publication is in any way politically motivated is beneath contempt.”
Others have expressed concern the guidance was released would make them “extremely unpopular” in their seats.
Another MP said: “I back Bridget, but if this guidance is published before the vote closes there’s no way I can vote for her. I’ve got a big LGBT community in my constituency and I’d never get their vote again.”
Her opponent in the upcoming deputy leadership contest, Lucy Powell, is understood to think now that MPs should be able to vote on the guidance because it will become law.
Shadow Women and Equalities Minister Mims Davies accused Phillipson of putting “her own career opportunities ahead of women’s rights.”
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